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HiSeq v4 Sequencing Workflow

HiSeq 2500 System User Guide

HiSeq v4 Sequencing Workflow

Prepare reagents for the run. Weigh reagents after preparation.

For reagent preparation information, see Reagent Preparation Steps on page

18.

Using the control software, enter run parameters.

When prompted, load all reagents for the run:

• Load SBS reagents for Read 1 and Read 2.

• For indexed runs, load indexing reagents.

• For paired-end runs, load paired-end reagents.

With a used flow cell on the instrument, confirm proper flow.

Prime SBS reagents and measure priming waste.

Load the clustered flow cell for sequencing. Confirm proper flow.

Start the sequencing run.

[Optional] After cycle 1, inspect the first base report, and then continue

The run continues as specified in run parameters.

When the run is complete, unload and weigh reagents.

Perform an instrument wash.

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