Directed Electronics equalizer 2500 Guia do Utilizador descarregar pdf (Página 110)

Post-Run Procedures

100

Part # 15035786 Rev. D

Perform a Maintenance Wash

A maintenance wash is required every 10 days or when switching between high output

and rapid modes, and is an option after a high output run. Illumina recommends

performing a maintenance wash after a high output run.

The Load Gasket screen opens with a maintenance wash every 10 days and when

switching from rapid mode to a high output mode. Replace the 10-port gasket in the front

manifold and the 8-port gasket in the back manifold before proceeding to the wash, even if

this screen does not appear.

A maintenance wash consists of washing the system with Tween 20 and ProClin 300.

User-Supplied Consumables

} Ethanol wipes

} 8 bottles, 250 ml (Corning, catalog # 430776)

} 10 tubes, 15 ml (Corning, catalog # 430052)

} [For Rapid Run mode] 1 Eppendorf tube per flow cell for washing the loading station

} Laboratory-grade water

} Tween 20 (Sigma-Aldrich, catalog # P7949)

} ProClin 300 (Sigma-Aldrich, catalog # 48912-U)

Prepare Wash Solution

Prepare 5 liters of maintenance wash solution for use with 1 instrument. The solution can

be used up to 3 times and stored for up to 30 days at room temperature.

NOTE

Dispose of wash solution in accordance with the governmental safety standards for your

region.

1 Prepare 250 ml of 10% Tween 20 by combining the following volumes, adding the

water first:

• Laboratory-grade water (225 ml)

• Tween 20 (25 ml)

2 Place a stir bar in an empty carboy that is at least 6 liters.

3 Combine the following volumes in the carboy, adding the water first:

1 2 ... 105 106 107 108 109 110 111 112 113 114 115 ... 153 154

Sem comentários

Directed Electronics equalizer 2500 Guia do Utilizador Página 110