Directed Electronics equalizer 2500 Guia do Utilizador descarregar pdf (Página 114)

Post-Run Procedures

104

Part # 15035786 Rev. D

Perform a Water Wash

A water wash is required after each sequencing run. After a high output run, you can

perform a maintenance wash instead.

If the instrument has been idle for 1 day or more, perform a water wash before beginning a

new sequencing run.

Illumina-Supplied Consumables

} Laboratory-grade water

User-Supplied Consumables

} 8 bottles, 250 ml (Corning, catalog#430776)

} 10 tubes, 15 ml (Corning, catalog#430052)

} Laboratory-grade water

} [For Rapid Run mode] 1 Eppendorf flip-top tube for each flow cell

Procedure

1 From the Welcome screen, select Wash | Water.

2 Select Yes to wash paired-end reagent positions. Otherwise, select No to wash

SBSreagent positions only. Select Next to proceed.

3 Load the instrument with laboratory-grade water as follows:

a Fill 8 SBSbottles with 20 ml laboratory-grade water.

b Fill 10 PEtubes with 10ml laboratory-grade water.

c [For Rapid Run mode] Fill the Eppendorf tube with 1ml laboratory-grade water.

4 Make sure that a used flow cell is loaded. Load a used flow cell, if necessary. Select

Next.

5 Perform a fluidics check:

a Select solution2 from the drop-down list. Accept the default pump values.

b Select Pump.

c Visually inspect the flow cell for bubbles passing through the lanes and leaks near

the manifolds.

1 2 ... 109 110 111 112 113 114 115 116 117 118 119 ... 153 154

Sem comentários

Directed Electronics equalizer 2500 Guia do Utilizador Página 114