Directed Electronics equalizer 2500 Guia do Utilizador descarregar pdf (Página 130)

Real-Time Analysis

120

Part # 15035786 Rev. D

NOTE

Quality scoring is based on a modified version of the Phred algorithm. For more

information, see en.wikipedia.org/wiki/Phred_quality_score.

Quality scoring calculates a set of predictors for each base call, and then uses the predictor

values to look up the Q-score in a quality table. Quality tables are created to provide

optimally accurate quality predictions for runs generated by a specific configuration of

sequencing platform and version of chemistry.

After the Q-score is determined, results are recorded in base call (*.bcl) files. For more

information, see Sequencing Output Files on page 121.

Q-Score Binning

Real-Time Analysis groups quality scores into specific ranges, or bins, and assigns a value

to each range. Q-score binning significantly reduces storage space requirements without

affecting accuracy or performance of downstream applications.

Q-score binning contributes to the efficiency of analysis processes and data transfer

requirements associated with the high throughput of the HiSeq 2500. The resulting *.bcl file

is smaller because the compression algorithms are able to compress the file more

effectively. Less data are written to the instrument computer and transferred to a network

location, making the file copy faster.

1 2 ... 125 126 127 128 129 130 131 132 133 134 135 ... 153 154

Sem comentários

Directed Electronics equalizer 2500 Guia do Utilizador Página 130