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Post-Run Procedures

Part # 15035786 Rev. D

Introduction

Post-run procedures include the removal and weighing of reagents, and an instrument

wash. A water wash is required after each run, with the option after a high output run to

perform a maintenance wash instead. Illumina recommends a maintenance wash.

Regular instrument washes ensure continued performance in the following ways:

} Flush any remaining reagents and sample from the fluidics tubing and sippers.

} Prevent salt accumulation and crystallization in the fluidics tubing and sippers.

} Prevent cross-contamination from the previous run, including cross-contamination

after switching modes.

NOTE

A water wash performed at the end of a run washes the system and checks fluidics. When

setting up a run, the software confirms that a water wash or maintenance wash has been

performed within the last 24 hours. A maintenance wash is required every 10 days. When 10

days elapse after the last maintenance wash, the software prompts you to perform a

maintenance wash.

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Directed Electronics equalizer 2500 Guia do Utilizador Página 108