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Stop and Resume a Run

HiSeq 2500 System User Guide

133

Stop and Resume a Run

Stopping a run might be necessary if the run was set up incorrectly, if the data quality is

bad, or if there is a hardware error. To resume a stopped run, make sure that you select the

appropriate normal stop options that allow the run to resume.

Stop Option Real-Time Analysis

Option

Able to Resume?

Normal Stop

(End of Lane\Chemistry)

Keep As Is Yes. The run resumes at the next chemistry or

imaging command.

Complete For Run No. The run cannot be resumed.

Complete For Read Yes. The run resumes at the beginning of the

next read.

Normal Stop

(End of Cycle)

Keep As Is Yes. The run resumes at the next cycle.

Complete For Run No. The run cannot be resumed.

Complete For Read Yes. The run resumes at the beginning of the

next read.

Immediate Stop No option No.

1 From the Run Overview screen, select Stop. The stop menu opens.

Figure 53 Stop the Run

2 Select 1 of the following stop options:

3 Select a stop option:

• Normal Stop (End of Lane\Chemistry)—Stops the run only after the current

chemistry or imaging command is complete, and then places the flow cell in a safe

state.

• Normal Stop (End of Cycle)—Stops the run after the current cycle is complete, and

then places the flow cell in a safe state.

• Immediate Stop—Stops the run without completing the current operation and does

not place the flow cell in safe state. You cannot resume a run that was stopped

using the immediate stop option.

4 Select from the following Real-Time Analysis options:

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